WebDec 1, 2024 · Macrophages are the preferential cell types to study various aspects of mycobacterial infection. Commonly used infection models for in-vitro studies are primary macrophages such as human monocyte derived macrophages (hMDMs) and macrophage like cell lines (THP-1). It is not clear if commercially available THP-1 cells can be used as … WebMar 4, 2024 · Abstract Results obtained showed that infection with HCMV prevented the death of THP-1 cells treated with DOX in both active and latent forms of infection. In the presence of mTOR inhibitors (rapamycin and Torin2), the sensitivity of the infected cells to DOX was restored. Rapamycin inhibited the expression of the HCMV protein IE1-p72 and …

CELL SEARCH SYSTEM -CELL BANK- (RIKEN BRC) [RCB1189 : THP-1]

Web3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take at … WebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically … e a r sheppard eastbourne

THP1 Cell Line - an overview ScienceDirect Topics

WebNov 1, 2014 · The THP-1 cell line is isolated from the peripheral blood of a 1-year old male patient suffering from acute monocytic leukemia [7]. This cell line has been widely used to study immune responses while cells are not only in the monocyte state but also in the macrophage-like state [8], [9]. THP-1 macrophages, when stimulated with LPS, expressed ... WebThe day of transfection, count the cells to determine culture density. Plate 1 x 10 5 cells per well in 0.5 ml of complete growth medium. Cell density should be 50~80% confluent on the day of transfection. For each well of cells to be transfected, dilute 0.5 μg of DNA into 100 μl of Opti-MEM® I Reduced Serum Medium without serum. WebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. … ct breastwork\\u0027s