Orc2 antibody drosophila
WebDrosophila Myb recognizes a highly conserved DNA sequence10,11, but the specific binding properties of the glycerol gradient fractions might be more complex than that of … WebApr 1, 1999 · We isolated mutations in Drosophila E2F and DP that affect chorion gene amplification and ORC2 localization in the follicle cells. In the follicle cells of the ovary, the ORC2 protein is localized throughout the follicle cell nuclei when they are undergoing polyploid genomic replication, and its levels appear constant in both S and G phases.
Orc2 antibody drosophila
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WebDec 1, 2024 · To analyze the ability of mutant proteins to interact with the rest of the complex, we immunoprecipitated ORC from obtained transgenic flies using Orc2 … WebApr 13, 2024 · Cell Research - Dietary cysteine drives body fat loss via FMRFamide signaling in Drosophila and mouse. ... Antibodies were used at the following dilutions: mouse anti-nc82 (1:100, DSHB), rabbit ...
WebContact Cusabio to Customize Orc2 Antibody, which reacts with Drosophila melanogaster (Fruit fly) and will validated for ELISA, WB (ensure identification of antigen). Call: 301-363-4651; ... Rabbit anti-Drosophila melanogaster (Fruit fly) Orc2 Polyclonal antibody: Uniprot No. Q24168: Target Names: Orc2: Alternative Names: WebOct 9, 2024 · Meier-Gorlin syndrome (MGS) is a rare autosomal recessive disorder characterized by microtia, primordial dwarfism, small ears and skeletal abnormalities. …
WebApr 13, 2024 · This study shows that Drosophila germ granules, which initially protect RNAs, subsequently become sites of selective mRNA degradation; this plasticity supports germ cell development. ... 50 μg/mL tRNA), embryos were incubated with primary antibody in PBHT overnight at 4°C with rocking. Primary antibodies used: 1:250 rabbit anti-CCR4 , 1:250 ... WebDec 14, 2000 · This pattern of staining was also observed using affinity-purified ORC2 antibody and in three Drosophila cell lines following either paraformaldehyde or methanol fixation (Fig. 2 b). ORC2 staining is remarkably similar to that of Xenopus ORC1, which is also absent from metaphase chromosomes but present on anaphase chromatids [33]. It …
WebJan 12, 2007 · We find that the developmental timing, epigenetic regulation, and Orc2 protein binding have been strictly conserved over 40 million years of evolution. We also present evidence that additional loci may be amplified in some species.
WebDrosophila melanogaster ORC PBS phosphate-buffered saline Initiation of eukaryotic DNA replication takes place at multiple sites in the genome. In yeast Saccharomyces cerevisiae, a six protein origin recognition complex (ORC) 1 binds to ARS consensus sequences in a sequence-specific manner ( 1 , 2 ). how do you pronounce productivityWebAbout 20% of the Orc2 subunit is eluted from the nuclei after an RNase treatment ... anti-rabbit IgG (Amersham) and Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes) were used as secondary antibodies. Drosophila cell culture extracts. Drosophila Schneider line 2 (S2) cells were maintained at 25°C in Schneider's Insect Medium ... phone number for att email supportWebPhosphospecific antibodies were generated using synthetic peptides corresponding to amino acids 112–120 (CELAKpTPQKS) and 221–230 (CPVGKEpTPSKR) of ORC2 for anti … phone number for att repair serviceWebThese antibodies target ORC2 in Human, Mouse and Rat samples. Our ORC2 polyclonal and monoclonal antibodies are developed in Rabbit and Rat. These antibodies have been … how do you pronounce prowessWebDec 19, 2002 · As the Drosophila Myb complex bound to both ACE3 and Ori-β in vitro, we tested whether the Myb complex directly interacted with Orc. Immunoprecipitation from … phone number for att prepaid customer serviceWebOct 8, 2010 · IP with Orc2 antibody followed by immunoblot with ORCA polyclonal antibody (pAb) showed the association of Orc2 with endogenous ORCA (molecular weight of ORCA ∼70kDa; Figure 1 D). Finally, immunoprecipitation with ORCA pAb in human cells clearly demonstrated the association of ORCA with ORC subunits ( Figure 1 E). phone number for att internetWebTo verify the phosphorylation at Thr-116 and Thr-226 of ORC2, we generated phosphoantibodies that specifically detected phospho-Thr-116 or phospho-Thr-226 of ORC2. The α-pT116 and α-pT226 antibodies recognized GST-ORC2 expressed in 293T cells (Fig. 1C). However, neither phosphoantibody detected mutant forms of the protein with Thr to … how do you pronounce proverbial