Lamp2b rvg
Tīmeklis2024. gada 20. sept. · 作者分别将含RVG-Lamp2b和T7-Lamp2b质粒转染入293T细胞，分离外泌体后再通过WB验证得到的RVG-exo和T7-exo都带有靶向配体。 随后通 … Tīmeklis2024. gada 31. janv. · Lamp2b was cloned with cDNA from C2C12 cells and XhoI and BspEI restriction sites were inserted after the signal peptide sequence together with …
Lamp2b rvg
Did you know?
Tīmeklis2015. gada 3. marts · This strategy has been applied to target exosome uptake by neurons by fusing a rabies viral glycoprotein (RVG) 3 peptide to the N terminus of … Tīmeklis2024. gada 10. janv. · A T7 peptide-decorated exosome (T7-exo) was produced by incorporation of T7 into the exosome membrane as a fusion protein of T7 and Lamp2b. As a control, rabies virus glycoprotein (RVG) peptide targeting brain neuron cells was incorporated into the exosome membrane. AMO-21 was loaded into the exosomes …
TīmeklispcDNA GNSTM-3-RVG-10-Lamp2b-HA质粒价格1200元，pcDNA GNSTM-3-RVG-10-Lamp2b-HA(爱迪基因 71294 )质粒图谱（map），质粒序列(Sequence)见下文，质粒 … Tīmeklis2024. gada 17. okt. · Lysosome-associated membrane glycoprotein 2b (Lamp2b) on the surface of the exosomes can be modified by genetic engineering without …
TīmeklisLAMP2. Lysosome-associated membrane protein 2 ( LAMP2 ), also known as CD107b ( C luster of D ifferentiation 107b) and Mac-3, is a human gene. Its protein, LAMP2, is … Tīmeklis2024. gada 15. nov. · 细胞外小囊泡（sEVsRVG） 的脑部神经特异性靶向是通过将sEVs膜蛋白lamp2b和神经靶向性的狂犬病毒糖蛋白衍生肽（RVG）融合递呈在 细胞外囊泡上，在细胞及动物水平验证了sEVsRVG-siRNA的抗病毒能力 图3： sEVsRVG-siRNA装载siRNA条件的优化及其在细胞水平上的抗病毒能力 图4：孕鼠模型证明 …
Tīmeklis2024. gada 8. janv. · Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome-lysosome fusion in human cardiomyocytes (CMs). Remarkably, LAMP-2B functions independently of syntaxin 17 (STX17), a protein that is essential for autophagosome-lysosome fusion in non-CMs. Instead, LAMP-2B interacts with …
Tīmeklis2011. gada 20. marts · Expression of the RVG and MSP Lamp2b constructs was confirmed by quantitative PCR (qPCR) analysis of transfected dendritic cells ( Fig. 1e … primed medical group trumbull ctTīmeklis2024. gada 21. okt. · The neuronal-targeted Lamp2b-RVG sEVs displayed higher levels of expression of Lamp2b compared to the native ones (Extended Data Fig. 1a,b) suggesting a good integration of Lamp2b-RVG at the sEV ... playing computer chessTīmeklis2024. gada 11. jūl. · Briefly, Lamp2b was cloned from the C2C12 mouse myoblast cell line with primers containing XhoI and BspEI restriction sites to enable subsequent … playing console games through vlcTīmeklis2024. gada 30. apr. · The transfection of the dendritic cells with the Lamp2b-RVG plasmid is necessary to obtain brain-targeted exosomes. Use a different targeting peptide to direct exosomes to other organ targets. Alternatively to produce non-targeted exosomes just replace the medium on day 4 with 3 mL medium supplemented with … playing computer games clipartTīmeklis2024. gada 4. aug. · A Lamp2b-based fusion protein can be used to display rabies viral glycoprotein (RVG), a neuron-specific targeting peptide, on the surface. Expression of Lamp2b-RVG fusion proteins in dendritic cells (DCs) displays RVG on the surface of DC-derived exosomes (Fig. 2 a). playing computer games is good for kidsTīmeklis2024. gada 2. maijs · RVG-Lamp2b-Modified Exosomes Specially Target the Injured Region To investigate the potential of RVG-Exos to specially deliver miR-124 to the injured region, we established a focal ischemia model. We performed photothrombosis in the somatosensory cortex ( Figure 2 A). primed medical products inc pg4-1201TīmeklisExpression of the RVG and MSP Lamp2b constructs was confirmed by quantitative PCR (qPCR) analysis of transfected dendritic cells Fig. 1e These modifications do not appear to affect the physical... playing computer audio on teams