Insulin-transferrin-selenium-ethanolamine
Nettet6. Saito Y, Yoshida Y, Akazawa T, Takahashi K, Niki E. 2003. Cell death caused by selenium deficiency and protective effect of antioxidants. J. Biol. Chem. 278(41):39428-34. Component g/L (100x) Recombinant human insulin 1.00 Recombinat human transferrin (Optiferrin) 0.55 Sodium Selenite 6.70E-04 Ethanolamine 0.20 … Nettet使用 gibco™ 胰岛素-转铁蛋白-硒 (its-g) 作为基础培养基补充剂，以减少培养细胞所需的胎牛血清 (fbs) 的量。its-g 溶液最初配合 rpmi 1640 和最低必需培养基 (mem) 使用，现在通常配合其他基础培养基使用，以在存在 2–4% fbs 的情况下支持多种细胞类型。
Insulin-transferrin-selenium-ethanolamine
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NettetIn the process of developing the medium, ethanolamine was found to be an essential growth factor for the hybridoma. Phosphoethanolamine at 10-fold higher concentration could substitute for ethanolamine. Long-term cultivation of the cells was achieved in the defined medium supplemented with insulin, transferrin, ethanolamine, and selenium. NettetProduct Description. ITSE + Albumin Animal-Free ™ is a recombinant blood-free insulin transferrin albumin 100x cell culture media supplement that also contains selenium and ethanolamine. ITSE + Albumin is intended to reduce or replace FBS or human serum in cell culture media.
NettetInsulin Transferrin Selenium Ethanolamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Nettet6. Saito Y, Yoshida Y, Akazawa T, Takahashi K, Niki E. 2003. Cell death caused by selenium deficiency and protective effect of antioxidants. J. Biol. Chem. 278(41):39428 …
Nettet8. apr. 2024 · We established the first continuous marine sponge cell line, originating from the boreal deep-sea sponge G. barretti. We previously reported that cells from multiple sponge species, including G ... NettetInVitria’s ITS, ITSE, and ITSE+A Animal-Free are recombinant, blood-free cell culture media supplements used to optimize cell growth. For optimal proliferation and performance, cells cultured must be supplied with a critical proteins and components:Insulin: promotes uptake of glucose and amino acids, facilitating cellular …
Nettet22. sep. 2024 · Abstract The potential ability to cultivate a recombinant immunoglobulin (IgG1)-producer line in Iscove’s Modified Dulbecco Medium (IMDM) as the base cultivation medium with the introduction of recombinant proteins and hydrolysates of non-animal origin was studied. Recombinant insulin, human serum albumin (produced by E. coli …
NettetIn this paper we report autonomous growth of the transfected CHO-K1 cell line expressing transferrin and IGF-1 in protein-free medium without the addition of exogenous growth factors. Growth rates and final cell densities of these cells were identical to that of the parent cell line CHO-K1 growing in insulin, transferrin, and selenium ... tti global phone numberNettetKeyword:'insulin-transferrin-selenium-ethanolamine' Showing 1-1 of 1 result for "insulin-transferrin-selenium-ethanolamine" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. Filter & Sort. All Photos (1) ITS Liquid Media Supplement (100×) Compare Product No. phoenix ctre downloadNettetITSE AF is a recombinant insulin and transferrin cell culture 100x media supplement. ITSE AF contains 4 components that are typically required for optimal cell growth in serum-free or reduced serum media. Insulin has cell signaling functions and promotes the uptake of glucose and amino acids. Transferrin is a universal source of iron for cells ... tti firearmsNettetACC-422 cells were grown in MEM media containing 1X Insulin-Transferrin-Selenium-Ethanolamine, 1% penicillin/streptomycin and 15% fetal bovine serum (FBS). Extracted molecule: total RNA: Extraction protocol: For each sample, RNA was collected using TRIzol and isolated using the miRNeasy mini kit (Qiagen 217004). tti ft worthNettetProduct Description. ITSE + Albumin Animal-Free ™ is a recombinant blood-free insulin transferrin albumin 100x cell culture media supplement that also contains selenium … ttigraas react fanficphoenix csd tax billNettetJournal: Cell Reports Methods Article Title: A culture platform to study quiescent hematopoietic stem cells following genome editing doi: 10.1016/j.crmeth.2024.100354 Figure Lengend Snippet: Article Snippet: Insulin-Transferrin-Selenium-Ethanolamine (ISTX) 1000x, Thermo Fisher Scientific , Cat# 51500-056. Techniques: Recombinant, … phoenix crown molding