WebBy combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP sequencing (ChIP-Seq) is a powerful method for identifying genome-wide DNA binding … http://www.cmctag.com/products/DYKDDDDK-FLAG-tag-rabbit-mab-Epitope-Tag-Antibody-ChIP-Grade-AT1748.html
(F1804) Monoclonal ANTI-FLAG(R) M2 antibody produced in mouse …
Webepitope (e.g., GFP; FLAG-tag, HA-tag, etc.) is fused with the protein–coding sequence of the transcription factor or other chromatin-associated protein of interest and produces a functional ... Result of peak-calling using the specific antibody in this experiment as the ChIP antibody in the parental, non-tagged cell line. This experiment ... WebDYKDDDDK Epitope Tag Antibodies. DYKDDDDK Epitope Tag antibodies, also known as FLAG® tagged antibodies, serve as universal detection reagents for FLAG® fusion proteins. The FLAG® tag is located at the N-terminus, C-terminus, or placed at an internal position of the recombinant protein, in order to easily characterize the protein of interest ... how do you cook vegetables in an air fryer
Anti-DYKDDDDK (FLAG®) tag Rabbit mAb - ChIP Grade
WebChIP was performed using Histone H3K4me1 Recombinant Rabbit Polyclonal Antibody (Cat. No. 710795, 5 µg) on sheared chromatin from 2 million Jurkat cells. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with optimized PCR primer pairs for the promoters of the active cFOS, beta-actin genes, and IRF1 ... WebApr 8, 2024 · The lysates were subsequently incubated with IgG or ChIP-grade antibody against FOXA2 at 4 °C overnight and then incubated with Protein A + G Agarose/Salmon Sperm DNA at 4 °C for 3 h. After washing with low salt and high salt buffer, elution, and reverse cross-linking, the DNA was added with EDTA, Tris pH 6.5, and proteinase K at … WebFusion proteins, in which a known peptide such as a His-Tag, GST-Tag, or DYKDDDDK (FLAG(R)) is cloned with the target of interest, were developed to simplify protein isolation. Since specific antibodies may not be available against the protein of interest, epitope tag antibodies are commonly used to detect protein expression. The epitope tag, … how do you cook veg in air fryer