Bin2 phosphorylation mutant

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August undefined, 2024

WebJul 22, 2009 · Plant Materials and Growth Conditions. Arabidopsis thaliana ecotype Columbia was used as WT control. BR-deficient mutant det2-1 and BR-insensitive mutant bin2-1 were obtained from Dr. Zhiyong Wang and Dr. Jun Zhao. WT and det2-1 seeds were surface-sterilized in 10% sodium hypochlorite and rinsed five times in sterile water. The … WebNov 11, 2024 · e, Western blot with the custom-made p-BIN2 antibody on BIN2-WT or BIN2-AA (T423A and S424A non-phosphorylable mutant)-expressing A2780 cells showed that the p-BIN2 antibody detected an expected ...

Phytochromes A and B Mediate Light Stabilization of BIN2 to …

WebBIN2 phosphorylation of RD26 is required for RD26 transcriptional activation on drought-responsive genes. RD26 overexpression suppressed the brassinazole (BRZ) insensitivity of BIN2 triple mutant bin2 bil1 bil2, … WebNov 9, 2024 · GSK3-like Kinases Negatively Regulate Salt Stress Tolerance. To determine if GSK3-like kinases function in the plant salt stress response, we assessed the salt sensitivity of a BIN2, BIL1, and BIL2 triple loss-of-function mutant (bin2-3bil1bil2, abbreviated 3b) (Yan et al., 2009).Seedlings of the 3b mutant had a significantly shorter primary root … first date casual outfits

Connected function of PRAF/RLD and GNOM in membrane …

WebNov 14, 2024 · In mature MMCs or meristemoids, BIN2 is expressed, is upregulated and initiates BASL polarization redundantly with the MAPK module. The BIN2–POLAR–BASL … WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced accumulation of BIN2 promotes the phosphorylation of BES1 in far-red light. BIN2 acts genetically downstream from PHYA to regulate photomorphogenesis under far-red light. WebBIN2 is a member of the GSK3-like kinase family, whose members can recognize substrates by either forming a stable protein complex with them or recognizing substrate epitopes that have been previously phosphorylated by an alternative protein kinase in a process known as priming phosphorylation. first date dinner conversation

Brassinosteroid signal transduction from cell-surface receptor …

The GSK3-like kinase BIN2 phosphorylates and …

WebApr 6, 2015 · AtSK21/BIN2 phosphorylation of ARF2 alleviates DNA binding and repressor activity of ARF2, ... Notably, mutant bin2-1 protein accumulates in greater quantities than wild-type AtSK21/BIN2 in plant cells (Peng et al., 2008). The BR biosynthetic inhibitor BRZ induces the accumulation of AtSK21/BIN2 in plant cells. WebIt was shown that the BIN2-catalyzed phosphorylation of BSK3 promotes the interactions of BSK3 with the BR receptor BRI1 (Brassinosteroid-Insensitive1) ... elimination of GSK3-like kinases resulted in an increased ratio of SE/CC whereas overexpression of a gain-of-function mutant variant bin2-1 slightly decreased the SE/CC ratio, ... eve cloakingWebJun 6, 2024 · Considering the BIN2 inhibitor bikinin greatly increased SA-induced gene expression (Figure 1 D–1F and Supplemental Figure 1 B–1D), we examined the functional involvement of BIN2 in SA signaling.The bin2-1 gain-of-function mutant displayed reduced sensitivity to SA in a bacterial growth assay (Figure 2 B). Similarly, the inhibitory effect of … eve clear cache

"WebNov 1, 2002 · It was hypothesized that, in the absence of BR signals, BIN2 is a constitutively active kinase that would phosphorylate several positive BR-signaling proteins, rendering … " - Bin2 phosphorylation mutant

Bin2 phosphorylation mutant

Histone deacetylase HDA6 enhances brassinosteroid …

WebAug 1, 2024 · BR-INSENSTIVE 2 (BIN2), a crucial negative regulator of brassinosteroid (BR) signaling, is induced by ABA and phosphorylates SnRK2s to enhance ABA signaling [5]. PP2Cs, such as ABI1 and ABI2, function as negative regulators of both BIN2 and SnRK2s, and thereby form a negative feedback regulatory loop in ABA signaling [12]. WebDec 20, 2012 · Mutant MBP-mBZR1 protein containing bzr1–1Dmuta- tion alsophosphorylated GST-BIN2.Consistent fastermobility mBZR1-CFPproteins im-munoblot analysis transgenicplants, bzr1–1D mutation ap- peared vitro-phosphorylatedMBP-mBZR1 protein hadlittle effect unphosphorylatedBZR1 fusion protein (Fig. 2B), suggesting …

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WebJan 10, 2024 · Bottom schematic shows BASL polarization requires MAPK-mediate protein phosphorylation and the plasma membrane-associated BRX proteins. Unknown regulators (orange) are anticipated to mediate BASL polarization. ... Genotyping results showed that these mutant plants are ... GSK3-like BIN2 kinases 12 and the BSL phosphatases 13, … WebAug 11, 2024 · BIN2 is an indispensable component of SA signaling; BIN2 interacts with and phosphorylates TGA3; SA-induced phosphorylation of TGA3 by BIN2 promotes the …

WebDec 29, 2024 · BIN2 mutant expression assay The native promoter-driven genomic BIN2 gene (4058 kb) and the corresponding mutated genes were fused with the YFP tag and transferred into a Col-0 background. The … WebOur genetic, biochemical, and physiological assays revealed that despite functional redundancy, BIN2 plays a dominant role among the three group II members in regulating …

WebApr 20, 2024 · In this study, we show that the GSK3 kinase BIN2 physically interacts with CO and the gain-of-function mutant bin2-1 displays late flowering phenotype through down-regulation of FT transcription. Genetic analyses show that BIN2 genetically acts upstream of CO in regulating flowering time. WebNov 14, 2024 · The BIN2–POLAR–BASL complex migrates to the cell cortex and polarizes before the ACD, thereby relieving the BIN2 inhibition on SPCH in the nucleus and attenuating the MAPK signalling in the...

WebNov 19, 2012 · BIN2 is a negative regulator in the BR pathway, which phosphorylates and inhibits BES1 and BZR1 (4, 48–50). BIN2 belongs to glycogen synthase kinase 3 …

WebBIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes … first date clothes for menhttp://genesdev.cshlp.org/content/28/15/1681.long eve clulowWebJul 11, 2002 · BIN2 encodes a member of the glycogen synthase kinase-3 (GSK3)-like kinases ( 14, 15 ). The increased kinase activity by the semidominant bin2–1 mutation and the phenotypes of transgenic plants … eve cloak trickWebJul 1, 2024 · BIN2, a loss-of-function mutant of BIN2 and its two closest homologs, was hyposensitive to ABA in primary root inhibition, ABA-responsive gene expression, and phosphorylating ABA Response Element Binding Factor (ABF) 2 fragment by in-gel kinase assays, suggesting that these GSK3-like kinases function as positive regulators in ABA … eve clan first date coversTo further identify the mechanism by which HY5 regulated BIN2 in the light to destabilize BZR1, we first checked whether HY5 regulated the mRNA and/or protein levels of BIN2. As shown in Supplementary Fig. 5, both the mRNA and protein levels of BIN2 were similar in hy5 and WT, suggesting that HY5 does not … See more To investigate the cross-talk between light and BR signaling, we performed yeast two-hybrid analyses and found that BIN2, the key negative … See more Next, we investigated whether HY5 could genetically interact with BIN2 by crossing hy5 into bin2-1, a gain-of-function mutant of BIN228. We found that the hypocotyls of double mutant … See more To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2-interacting sites in the HY5 LZ region. As shown in Fig. 5a, changing HY5 Leu137 (L137) to Asp (named HY5-M) … See more Previous reports have suggested that HY5 and BIN2 have similar effects on BZR1 protein accumulation20,27. To gain further insight into their … See more eve clocloWebAug 25, 2016 · The regulatory mechanisms by which BRs and other signals regulate BIN2 activity and stability remain unclear. Although in BR signaling it is reported that BIN2 can be regulated by dephosphorylation of the Y200 residue (), the functions of many other phosphorylation sites remain unknown.In addition, overexpression of Y200 … first date dinner outfit ideas